Spectroscopic and thermodynamic approach to the interaction of nonperipherally substituted cationic phthalocyanines with calf thymus (CT)-DNA


Tuncer S., Özçeşmeci İ. , Sesalan B. Ş. , Kalkan Burat A.

TURKISH JOURNAL OF CHEMISTRY, cilt.42, ss.274-295, 2018 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 42 Konu: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.3906/kim-1705-59
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Sayfa Sayıları: ss.274-295

Özet

Novel, nonperipherally tetrasubstituted cationic metal-free and metallophthalocyanines (Zn, In) were synthesized in the present study. The binding constants, the disappearance of quenching effect of all cationic phthalocyanines on the fluorescence intensity of SYBR Green-disodium salt of deoxyribonucleic acid (DNA) from calf thymus complex, and the changes in T-m of double helix DNA with thermal denaturation profile were investigated by UV-Vis and fluorescence spectrophotometric methods. To investigate the spontaneity of the reactions between DNA and novel quaternized phthalocyanines in buffer, thermodynamic parameters were employed.