A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation


Mozioglu E., Akgoz M., Tamerler C., KOCAGÖZ Z. T.

TURKISH JOURNAL OF BIOLOGY, vol.38, no.1, pp.125-129, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 1
  • Publication Date: 2014
  • Doi Number: 10.3906/biy-1308-7
  • Journal Name: TURKISH JOURNAL OF BIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.125-129
  • Istanbul Technical University Affiliated: Yes

Abstract

A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison.