New metal based drugs: Spectral, electrochemical, DNA-binding, surface morphology and anticancer activity properties

Cesme M., Gölcü A., Demirtas I.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, cilt.135, ss.887-906, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 135
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.saa.2014.06.144
  • Dergi Adı: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.887-906
  • Anahtar Kelimeler: Metal based drugs, Piroxicam, Electroanalysis, DNA binding, xCELLigence, SEM, NONSTEROIDAL ANTIINFLAMMATORY DRUGS, X-RAY, INTESTINAL TUMORS, SPECTROSCOPIC CHARACTERIZATION, STRUCTURAL-CHARACTERIZATION, VOLTAMMETRIC DETERMINATION, CYCLOOXYGENASE INHIBITORS, MOLECULAR-STRUCTURE, TERNARY COMPLEXES, CRYSTAL-STRUCTURE
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

The NSAID piroxicam (PRX) drug was used for complex formation reactions with Cu(II), Zn(II) and Pt(II) metal salts have been synthesized. Then, these complexes have been characterized by spectroscopic and analytical techniques. Thermal behavior of the complexes were also investigated. The electrochemical properties of all complexes have been investigated by cyclic voltammetry (CV) using glassy carbon electrode. The biological activity of the complexes has been evaluated by examining their ability to bind to fish sperm double strand DNA (FSFSdsDNA) with UV spectroscopy. UV studies of the interaction of the PRX and its complexes with FSdsDNA have shown that these compounds can bind to FSdsDNA. The binding constants of the compounds with FSdsDNA have also been calculated. The morphology of the FSdsDNA, PRX, metal ions and metal complexes has been investigated by scanning electron microscopy (SEM). To get the SEM images, the interaction of compounds with FSdsDNA has been studied by means of differential pulse voltammetry (DPV) at FSdsDNA modified pencil graphite electrode (PGE). The decrease in intensity of the guanine oxidation signals has been used as an indicator for the interaction mechanism. The effect of proliferation PRX and complexes were examined on the HeLA and C6 cells using real-time cell analyzer with four different concentrations. (C) 2014 Published by Elsevier B.V.