This paper presents a comprehensive framework for determining the interaction between transport, land-use and environmental impacts, where the developed concepts were tested using a case study. As the study area, two bridges connecting Europe and Asia and the planned third bridge was selected, where the constructed transportation infrastructures had drastically changed the land-use profile and still have negative impacts on environment. After exploring the interaction systematically and identifying the land cover/use pattern specific for the Istanbul Metropolitan Area, possible alternatives routes for the new bridge were by means of multi-criteria decision making approach and potential prediction of impacts were performed. The former land-use, transport infrastructure data were integrated with Landsat TM satellite images retrieved in 1987, 1997 and 2005. In order to identify the pattern of interaction, the land use analyses of the Asian side of the Istanbul Metropolitan area were compared within a selected box buffering the current and planned highways. For the planned third bridge alternative scenarios were selected, where alternatives were environmental friendly, cost-efficient and compatible with the current infrastructure. The results show that, easy accessibility caused by the development in transportation infrastructures created an attraction in this region and urban areas expanded rapidly. By means of the shortly described methodology and achieved results, the proposed framework aids authorities and decision-makers to better facilitate sustainable transportation.