Immobilized glucose oxidase biofuel cell anode by MWCNTs, ferrocene, and polyethylenimine: Electrochemical performance

Bahar T., Yazici M. S.

ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING, vol.13, no.1, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.1002/apj.2149
  • Journal Name: ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: bioanode, enzyme fuel cell, enzyme immobilization, glucose oxidase, MEDIATED ELECTRON-TRANSFER, POLYMER, INVERTASE, BIOANODES, BEHAVIOR, FILMS
  • Istanbul Technical University Affiliated: No

Abstract

Ferrocene-functionalized polyethylenimine and multiwalled carbon nanotubes were attached covalently by glutaraldehyde onto a carbon cloth to develop an immobilized enzyme (glucose oxidase) electrode for biofuel cell applications. Developed enzymatic anode was characterized by electrochemical methods to determine electrochemical performance. Anodic open-circuit potential was measured as within 0-20 mV range. Cyclic voltammetry showed anodic peak for glucose oxidation around 400-600 mV (vs. sat. Ag/AgCl) varying with scan rate. An enzyme fuel cell with 2.5 mg/cm(2) glucose oxidase-loaded bioanode and 0.70 mg/cm(2) Pt-loaded cathode attached to Nafion 115 membrane has provided around 2.5 mA/cm(2) current density at short-circuit conditions. Enzymatic kinetic parameters of prepared anode were determined by electrochemical methods that surprisingly indicated less K-M (i.e., better substrate affinity) than that of determined by conventional enzymatic methods. Enzymatic stability determined by electrochemical methods moreover indicated longer enzyme half-life.