Continuous measurement of temperature profiles on the surface of the human body offers various kinds of information valuable for clinical diagnosis and as a useful guide to take suitable action. In this article, the behavior of a newly developed temperature sensing fabric (TSF) over extended periods of time in a practical application and its comparison with a reference temperature sensor was investigated. The performance of the TSF was analyzed by measuring the human body skin temperature under steady-state and dynamic conditions. A dedicated interface was built in the LabVIEW environment to measure and record the temperature readings of the TSF and reference temperature sensor simultaneously. The reference and TSF sensors both followed exactly the same trends and experienced the same type of movement artifacts.