Biomarkers of oxidative stress, antioxidant defence and inflammation are altered in the senescence-accelerated mouse prone 8

Bayram B., NIKOLAI S., HUEBBE P., Özçelik B., GRIMM S., GRUNE T., ...More

AGE, vol.35, no.4, pp.1205-1217, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.1007/s11357-012-9448-0
  • Journal Name: AGE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1205-1217
  • Keywords: SAMP8, Accelerated ageing, Stress response, Proteasomal activity, Ascorbic acid, Tocopherol, VITAMIN-E, ALPHA-TOCOPHEROL, HUMAN LONGEVITY, BRAIN, LIVER, MICE, PEROXIDATION, MELATONIN, GENE, AGE
  • Istanbul Technical University Affiliated: Yes

Abstract

In this study we compared biomarkers of oxidative stress, stress response, antioxidant defence and inflammation between mice (n = 10 per group, female, 7 months old) with an accelerated (SAMP8) and a normal ageing phenotype (SAMR1). As compared to SAMR1 mice, SAMP8 mice exhibited higher levels of lipid peroxides and protein carbonyls as well as a lower activity of the proteasomal subunit beta-5. Furthermore, heme oxygenase-1 and paraoxonase-1 (PON-1) status was lower in SAMP8 mice indicating impaired stress response. Biomarkers of inflammation such as C-reactive protein and serum amyloid P were elevated in SAMP8 mice. Interestingly, impaired stress response and increased inflammation in SAMP8 mice were associated with elevated concentrations of ascorbic acid and alpha-tocopherol in the liver. An age-dependent increase in hepatic vitamin E and a decline in PON-1 gene expression were also observed in aged compared to young C57BL/6 mice.