Evolutionary Engineering of Yeast


Alkim C., Turanli-Yildiz B., Çakar Z. P.

YEAST METABOLIC ENGINEERING: METHODS AND PROTOCOLS, vol.1152, pp.169-183, 2014 (Refereed Journals of Other Institutions) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1152
  • Publication Date: 2014
  • Doi Number: 10.1007/978-1-4939-0563-8_10
  • Title of Journal : YEAST METABOLIC ENGINEERING: METHODS AND PROTOCOLS
  • Page Numbers: pp.169-183

Abstract

Evolutionary engineering is an inverse metabolic engineering strategy which is based on increasing genetic diversity and screening large populations for desired phenotypes. This strategy is highly advantageous in certain situations over rational metabolic engineering approaches, since there is little or no requirement of detailed genetic background information for the trait of interest. Here, we describe the experimental methodology for selecting stress-resistant yeast strains via evolutionary engineering approach by either serial batch or chemostat cultivations.