The genus populus provides creamy white, lustrous, lightweight fibers that grow on seeds. Since populus fibers obtained from populus nigra italica are too short (0.25-0.42 cm) to be spun into yarns, populus fibers were spray coated with dimethylacetamide/Spandex solution in order to form non-woven structures. In this study, the oil absorption capacity of non-woven populus fiber pads was investigated and compared to non-woven long cotton fiber and non-woven short cotton fiber pads. The hollow structure and the oleophilic properties of populus fibers make them promising and sustainable oil absorbent candidates. The average short-term rose oil absorption capacity of non-woven populus fiber pads is 32.96798 g/g, while the long-term rose oil absorption capacity of non-woven populus fiber pads is 34.52854 g/g. Furthermore, the toluene absorption capacity of non-woven populus fiber pads is on average 23.16986 g/g for the short-term test and 24.01588 g/g for the long-term test. Moreover, non-woven populus fiber pads preserve 81.75% of rose oil even after remaining in water for 45 minutes.