Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers


TIRKES S., TOPPARE L., ALKAN S., BAKIR U., ONEN A., Yagci Y.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.30, sa.2, ss.81-87, 2002 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 30 Konu: 2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0141-8130(02)00011-9
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Sayfa Sayıları: ss.81-87

Özet

Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polyprrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran). (1) and (3): and poly(pyrrole-graft-polystyrene polytetrahydrofuran), (2). The kinetic parameters V-max and K-m, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes. p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate. Sodium p-toluene sulfonate (NaPTS). and sodium dodecyl sulfate. sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high K-m value (59.9 mM) of enzyme immobilized in PPy was decreased aria immobilization in graft copolymer matrices of pyrrole, V-max, which was 2.25 mM/min for pure PPy, as found as 4.71 mM/min for compound (3). (C) 2002 Elsevier Science B.V. All rights reserved.