Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers


TIRKES S., TOPPARE L., ALKAN S., BAKIR U., ONEN A., Yagci Y.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.30, no.2, pp.81-87, 2002 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 2
  • Publication Date: 2002
  • Doi Number: 10.1016/s0141-8130(02)00011-9
  • Title of Journal : INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Page Numbers: pp.81-87

Abstract

Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polyprrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran). (1) and (3): and poly(pyrrole-graft-polystyrene polytetrahydrofuran), (2). The kinetic parameters V-max and K-m, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes. p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate. Sodium p-toluene sulfonate (NaPTS). and sodium dodecyl sulfate. sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high K-m value (59.9 mM) of enzyme immobilized in PPy was decreased aria immobilization in graft copolymer matrices of pyrrole, V-max, which was 2.25 mM/min for pure PPy, as found as 4.71 mM/min for compound (3). (C) 2002 Elsevier Science B.V. All rights reserved.