Electron microprobe analyses of gold and associated ore minerals as well as stable isotope analyses of sulphide and carbonate minerals were performed in order to determine the metal and fluid sources and temperature of the mineralizing systems to better understand the genesis of the Atud gold deposit hosted in the metagabbro-diorite complex of Gabal Atud (Central Eastern Desert, Egypt). The gold can be classified as electrum (63.6-74.3wt.% Au and 24.6-26.6wt.% Ag) and is associated with arsenopyrite and As-bearing pyrite in the main mineralization (gold-sulphides) phase within the main mineralized quartz veins and altered host rocks. Based on the arsenopyrite geothermometer, As-contents (29.3-32.7 atom%) in arsenopyrite point to deposition in the Log integral(S2) and T ranges of -10.5 to -5.5 and 305-450 degrees C, respectively, during the main mineralizing phase. Based on the S-34 isotopic compositions of the sulphides, they are originated from magmatic fluids in which the sulphur is either sourced directly from magma or remobilized from the magmatic rocks (gabbroic rocks). On the other hand, calcite formed from fluids having mainly magmatic mixed with variable metamorphic signatures based on its C-13 and O-18 values. This work concluded that the gold-bearing ores at Atud deposit have magmatic sources leaching from the country intrusive rocks during water/rock interactions then remobilized during a metamorphic event. Therefore, the Atud gold deposit is classified as an intrusion-related gold deposit, in which the gabbro-diorite host intrusion acted as the source of metals which were mobilized and deposited as a result of the effects of NW-SE shearing.