HUMAN HEPATOMA-CELLS EXPRESSING HLA CLASS-I MOLECULES STIMULATE PRIMARY RESPONSES OF PURIFIED CD8+ T-LYMPHOCYTES

PAROLI M., CARLONI G., FRANCO A., DEPETRILLO G., ALFANI E., BARNABA V.

RESEARCH IN VIROLOGY, vol.144, no.4, pp.327-332, 1993 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 144 Issue: 4
  • Publication Date: 1993
  • Journal Name: RESEARCH IN VIROLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.327-332
  • Istanbul Technical University Affiliated: No

Abstract

In the present study, we investigated the ability of major histocompatibility complex (MHC) class-I+ human hepatoma cell lines to induce primary proliferative responses of purified allogeneic CD8+ T lymphocytes. We found that HA22T/VGH and Li7A, but not HepG2 cells induced significant proliferation of CD8+ T cells and that these responses were depedent on class I molecule expression. In blocking experiments carried out to identify the costimulatory signals involved, we found that anti-ICAM1 monoclonal antibodies drastically inhibited CD8+ T-cell proliferative responses. These findings suggest that transformed hepatocytes expressing HLA class I molecules may participate in anti-tumour immunosurveillance by the direct induction of cytotoxic T-cell responses through ICAM1-mediated adhesive interaction.