loTs and Loop-Mediated Isothermal Amplification- Based Biosensing using Cloud-Enabled Features


Bayindir T., Degirmenci M., Ergenç A. F., Elitas M.

2022 Medical Technologies Congress, TIPTEKNO 2022, Antalya, Turkey, 31 October - 02 November 2022 identifier identifier

  • Publication Type: Conference Paper / Full Text
  • Doi Number: 10.1109/tiptekno56568.2022.9960156
  • City: Antalya
  • Country: Turkey
  • Keywords: biosensor, loop-mediated isothermal amplification, mobile, smartphone, analytical, cloud
  • Istanbul Technical University Affiliated: Yes

Abstract

© 2022 IEEE.Internet-of-Things technology (IoTs) have accelerated biosensor applications in all fields. Loop-mediated isothermal amplification (LAMP)-based biosensor technologies in conjunction with smartphone detection have been adequate to cover the demands of mobile diagnostics. The ease of use, affordability, portability, high sensitivity, flexibility, and specificity demands of point-of-care detection can be achieved by low-cost electronic components, 3-dimensional printing technologies, capturing images of calorimetrically detected readouts made our system a promising approach for real-time point-of-detection in the field. In this study, we implemented a cloud service to our LAMP-based biosensor. We previously performed bacteria detection using colony-based LAMP device and now distributed the optical readouts of the assay using smartphones. We transferred the obtained image and results of the assays through cloud. Our user-friendly interface simplifies the data processing, it directly digitized the readouts and eliminates the need of data interpretation.