Immobilization of yeast cells in several conducting polymer matrices

BALCI, Z; AKBULUT, U; TOPPARE, L; ALKAN, S; BAKIR, U; Yagci, Yusuf

doi:10.1081/ma-120013259

Immobilization of yeast cells in several conducting polymer matrices

Atıf İçin Kopyala

BALCI Z., AKBULUT U., TOPPARE L., ALKAN S., BAKIR U., Yagci Y.

JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, cilt.39, sa.3, ss.183-197, 2002 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 39 Sayı: 3
Basım Tarihi: 2002
Doi Numarası: 10.1081/ma-120013259
Dergi Adı: JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.183-197
İstanbul Teknik Üniversitesi Adresli: Evet

Özet

Immobilization of yeast cells (Saccharomyces cerevisiae) in different polymer matrices was performed by constant potential electrolysis. These matrices were polypyrrole (PPy); poly(methyl methacrylate)/polypyrrole (,PMMA/PPy) and thiophene-capped poly(methyl methacrylate)/ polypyrrole (TPMMA/PPy). The characterization of PMMA/PPy copolymer was achieved by Fourier-transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). The invertase activity of immobilized yeast cells was determined. Optimum temperature, Michaelis-Menten constants and maximum reaction rates of the enzyme electrodes were compared with those of free yeast cells. The operational and storage stabilities of three different immobilization systems were analyzed.