Sorting of micron-sized particles using holographic optical Raman tweezers in aqueous medium


Parlatan U., Basar G., Başar G.

JOURNAL OF MODERN OPTICS, vol.66, no.2, pp.228-234, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 66 Issue: 2
  • Publication Date: 2019
  • Doi Number: 10.1080/09500340.2018.1515378
  • Title of Journal : JOURNAL OF MODERN OPTICS
  • Page Numbers: pp.228-234

Abstract

We have constructed a holographic optical tweezers system combined with Raman spectroscopy to sort trapped particles. Our software automatically moves the trapped objects to the measurement positions to obtain individual Raman signals from multiple trapped particles. We performed the sorting by comparing their spectra with the previously measured training dataset using the correlation coefficients. We used yeast cells and polystyrene beads as test particles. This study aims to show that biological particles can be separated using single cell analysis with combined holographic optical tweezers and Raman spectroscopy system.