In this study, a molecularly imprinted polymer (MIP) was synthesized by electrochemical polymerization and used to construct an electrochemical sensor for determination of meldonium (MEL) selectively for the first time. The polymer film was generated by using o-phenylenediamine (o-PD) as a monomer on the surface of carboxylic acid functionalized multiwalled carbon nanotube (MWCNT) modified pencil rod electrode in the presence of MEL as a template. MEL imprinted (MEL-imp) and non-imprinted (N-imp) polymer films and coated electrodes were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), profilometry, electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Voltammetric measurements were carried out in a ferrocyanide/ferricyanide redox probe solution for MEL-imp and N-imp electrodes in the presence and absence of template molecule. The decrease in peak current of redox probe was linear with the concentration of MEL in the range of 0.1-5 mu g/mL and the limit of detection (3 s/b) was found to be 0.066 mu g/mL under optimized experimental conditions. The proposed sensor was successfully applied for selective determination of MEL in human urine sample with long term stability and good reproducibility.