Determination of khellin and visnagin in Ammi visnaga fruits by capillary electrophoresis

GUNAYDIN K., Erim F. B.

JOURNAL OF CHROMATOGRAPHY A, cilt.954, ss.291-294, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 954
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0021-9673(02)00168-1
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY A
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.291-294
  • Anahtar Kelimeler: Ammi visnaga, plant materials, pharmaceutical analysis, khellin, visnagin, coumarin, furanochromones, PERFORMANCE LIQUID-CHROMATOGRAPHY, SOLID-PHASE EXTRACTION, ROOT CULTURES
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

A new, simple and rapid capillary electrophoresis method was developed for the identification and quantitative determination of two medically active constituents-khellin and visnagin-in the extracts of Ammi visnaga fruits. Micellar electrochromatographic separation of khellin and visnagin was carried out using 10 mmol/l borate, 50 mmol/l sodium dodecylsulfate, 25% (v/v) acetonitrile at pH 9 as running buffer. Ammi visnaga fruits were extracted with methanol and the extracts were directly injected without any purification and pre-separation processes. Coumarin was used as internal standard for quantitation and the limits of detection for khellin and visnagin were 2.36 and 1.97 mug/ml, respectively using UV detection at 245 nm. (C) 2002 Elsevier Science B V. All rights reserved.