Viticulture is very sensitive to water stress, which is critical and influenced by all environmental factors, relating to the crop quality and productivity of vineyards. In this study, water stress was examined in veraison and harvest stages for nine different species with spectroradiometric measurements. Leaf water potential (LWP) values from field measurements and original spectra-based (OSB) and continuum removed spectra-based (CRSB) curves were analyzed with correlation and regression analysis to find the highest related wavelengths. The analysis was done for both specific dates of field measurements (i.e. 08.08.2012 and 06.09.2012) and also in aggregate i.e. all measured data. The specific date wavelength-based analysis revealed the “red edge region” as a major water stress indicator. The highest correlated wavelength was found to be 684 nm of CRSB curves with R=0.988. For the aggregate wavelength-based water stress analysis, the “violet and green regions” were identified as the best indicators. The highest correlated wavelength was found to be 410 nm of OSB curves with R=0.820. Furthermore, the Analysis of Variance (ANOVA) testing indicates that the results are significant at relatively high confidence levels. The spectral-based method performed in this study provides fast, flexible, and non-destructive water stress measurements of grapevines when compared to classical methods.