Electron donation mechanisms of beta-carotene as a free radical scavenger


Lee J., Ozcelik B., Min D.

JOURNAL OF FOOD SCIENCE, cilt.68, sa.3, ss.861-865, 2003 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Konu: 3
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1111/j.1365-2621.2003.tb08256.x
  • Dergi Adı: JOURNAL OF FOOD SCIENCE
  • Sayfa Sayıları: ss.861-865

Özet

The free radical scavenging ability of beta-carotene was studied using free radical 2,2-diphenyl-1- picryl-hydrazyl (DPPH). As the concentration of beta-carotene in acetone increased, the absorbance of DPPH at 517 nm decreased, which indicates that beta-carotene scavenges free radicals. It was shown that beta-carotene does not work as an antioxidant in soybean oil. The NMR spectrum of DPPH with BHA in CDCl3 showed a new peak at 5.0(d) was formed and the peak at 6.6(d) from BHA was split into 2 peaks, which may be due to the hydrogen atom donation from BHA to DPPH. The NMR spectra of DPPH with beta-carotene did not change during reaction and no peak was formed.