Electron donation mechanisms of beta-carotene as a free radical scavenger

Lee J., Ozcelik B., Min D.

JOURNAL OF FOOD SCIENCE, vol.68, no.3, pp.861-865, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 68 Issue: 3
  • Publication Date: 2003
  • Doi Number: 10.1111/j.1365-2621.2003.tb08256.x
  • Title of Journal : JOURNAL OF FOOD SCIENCE
  • Page Numbers: pp.861-865


The free radical scavenging ability of beta-carotene was studied using free radical 2,2-diphenyl-1- picryl-hydrazyl (DPPH). As the concentration of beta-carotene in acetone increased, the absorbance of DPPH at 517 nm decreased, which indicates that beta-carotene scavenges free radicals. It was shown that beta-carotene does not work as an antioxidant in soybean oil. The NMR spectrum of DPPH with BHA in CDCl3 showed a new peak at 5.0(d) was formed and the peak at 6.6(d) from BHA was split into 2 peaks, which may be due to the hydrogen atom donation from BHA to DPPH. The NMR spectra of DPPH with beta-carotene did not change during reaction and no peak was formed.