Omerli Reservoir is one of the major drinking water reservoirs of Greater Metropolis Istanbul, providing 40% of the overall water demand. Istanbul where is one of the greatest metropolitan areas of the world with a population over 10 million and a rate of population increase about twice that of Turkey. As a result of population growth and industrial development, Omerli watershed is highly affected by the wastewater discharges from the residential areas and industrial plants. The main objective of this study is to investigate the temporal assessment of the land-use/cover of the Omerli Watershed and the water quality changes in the Reservoir. It is not possible to adequately control urbanization and other pollution sources affecting the water quality. Responses of these detrimental effects are due to rapidly increasing population, unplanned and illegal housing, and irrelevant industries at the protection zones of the watershed, together with insufficient infrastructure. The study is focused on the assessment of urbanization in relation to land use and water quality using Remote Sensing (RS) and Geographic Information Systems (GIS) techniques for all the four protection zones of the Reservoir and a time variant analyzing model is obtained. IRS-1C LISS and IRS-1C PAN, LANDSAT-5 TM satellite data of 1997, 1998, 2000, 2001 and 2006 are analyzed by confirmation through the ground truth data. RS data have been transferred into UTM coordinate system and image enhancement and classification techniques were used. Raster data were converted to vector data that belongs to study area to analyze in GIS for the purpose of planning and decision-making on protected watersheds.