Preparation of calcium phosphate/carboxymethylcellulose-based bone cements

Guben E., Arici S., Bayir D., Bozdağ S. E. , EGE D.

BIOINSPIRED BIOMIMETIC AND NANOBIOMATERIALS, vol.9, no.3, pp.155-163, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 3
  • Publication Date: 2020
  • Doi Number: 10.1680/jbibn.19.00053
  • Page Numbers: pp.155-163


In this study, novel bone substitutes were prepared based on a powder phase composed of tetracalcium phosphate, dicalcium phosphate dihydrate and calcium sulfate dihydrate and a liquid phase composed of carboxymethylcellulose (CMC), citric acid and gelatin. Samples were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical testing, swelling-degradation studies and cell culture studies. FTIR results showed that CMC and calcium phosphate interacted through electrostatic forces and hydrogen bonding. After incubation in phosphate-buffered saline for 28 days, hydroxyapatite formation was distinguished by way of SEM and XRD analysis. Mechanical test results revealed that the compressive modulus was up to 1.050 +/- 0.071 GPa and that the compressive strength was up to 1.680 +/- 0.023 MPa. Cell culture studies indicated that the samples were biocompatible and may be suitable for replacing cancellous bone and supporting new bone formation.