Double fluorescence assay via a beta-cyclodextrin containing conjugated polymer as a biomimetic material for cocaine sensing

Arslan M., Sengel T. Y., Guler E., GÜMÜŞ Z. P., ALDEMİR E., Akbulut H., ...Daha Fazla

POLYMER CHEMISTRY, cilt.8, sa.21, ss.3333-3340, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 21
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1039/c7py00420f
  • Dergi Adı: POLYMER CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3333-3340
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

A double fluorescence mu-well assay that exploits a novel conjugated polymer containing cyclodextrin (CD) as the key component is reported. For the construction of the cocaine bioassay, poly(p-phenylene) with CD units in the main-chain and poly(ethylene glycol) side chains (PPP-CD-g-PEG) was first prepared by Suzuki coupling polymerization and coated on each well as a biomimetic material. Although the poly-phenylene backbone is responsible for the fluorescence properties without an additional fluorophore, PEG and CD provide water solubility and selective complexation with cocaine, respectively. A cocaine antibody was used as a secondary recognition compound after labelling with quantum dots (QDs). Most notably, we show that the two-color fluorescence nature of the assay facilitates double measurement from the same sample and the described strategy can be adapted to various sensing systems.