Expression and functional analysis of glutamate synthase small subunit-like proteins from archaeon Pyrococcus horikoshii

Dincturk H. B., Cunin R., Akce H.

MICROBIOLOGICAL RESEARCH, cilt.166, sa.4, ss.294-303, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 166 Sayı: 4
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.micres.2010.03.006
  • Dergi Adı: MICROBIOLOGICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.294-303
  • İstanbul Teknik Üniversitesi Adresli: Evet

Özet

Glutamate synthase, glutamine a-ketoglutarate amidotransferase (often abbreviated as GOGAT) is a key enzyme in the early stages of ammonia assimilation in bacteria, algae and plants, catalyzing the reductive transamidation of the amido nitrogen from glutamine to a-ketoglutarate to form two molecules of glutamate. Most bacterial glutamate synthases consist of a large and small subunit. The genomes of three Pyrococcus species harbour several open reading frames which show homology with the small subunit of glutamate synthase. There are no open reading frames which may be coding for a large subunit responsible for the glutamate formation in these pyrococcal genomes.