Although pharmacies play an important role in providing information and advice on health issues to lower income people in developing countries, the distribution of urban pharmacies is not balanced with respect to population distribution in some of the districts of Istanbul. This situation can severely limit the accessibility of pharmacy services to the people with lower socio-economic background. A multiple regression analysis is used in order to find out the factors which affect the spatial distribution of pharmacies in Istanbul. The number of pharmacies in the districts considered is taken as the dependent variable and the number of hospital beds, physicians, population, employment, income, number of educated people and distance to the CBD are taken as independent variables. According to the results, the population of the districts and the number of educated people, physicians and hospital beds are the most important factors affecting the location of pharmacies in Istanbul. The other variables are not found significant in affecting the location of pharmacies at the metropolitan level. Suggestions are made for better distribution of pharmacies and for further research.