This study has been carried out to optimise the silver (Ag) content of the coating synthesised on commercially pure titanium (Cp-Ti, Grade 4) for biomedical applications by micro-arc oxidation (MAO) process. The MAO process has been conducted in electrolytes containing silver acetate (AgC2H3O2) at different concentrations between 0 and 0.002 mol L-1. When compared to the base electrolyte, coatings synthesised in >= 0.001 mol L-1 AgC2H3O2 added electrolytes exhibited an antibacterial efficiency of 99.8% against Staphylococcus aureus (S. aureus). Detailed examination revealed that the presence of 0.01 mol L-1 AgC2H3O2 in the electrolyte resulted in incorporation of 1.4 wt-% Ag into fabricated coating consisting mainly of outer hydroxyapatite (HA) and inner titanium oxide (TiO2) layers. In comparison to the Ag-free coating, 1.4 wt-% Ag in the coating lowered the proliferation of SAOS-2 cells, which still tended to grow at a relatively low rate with increasing culturing time.