In this study, the functionalization of nanofibers by incorporating different particles and additives into the polymeric matrix for developing ultraviolet protective materials was investigated. Titanium dioxide was selected because of its known characteristic of being a widely used material for obtaining ultraviolet protection. Besides, 1,3,7-Trimethyl xanthine, which was used previously in medical applications to decrease the harm of ultraviolet radiation on DNA, was used to see its potential in obtaining ultraviolet protective nanofibers. By using these two different additives in different concentrations, functionalized polyamide 6.6 nanofibers were produced, their ultraviolet protection property and ratings were recorded, and the results were evaluated comparatively. Addition of titanium dioxide in all concentrations significantly improved ultraviolet protection of polyamide 6.6 nanofibers for both UVA- and UVB-type radiations. Moreover, ultraviolet protection factor value of polyamide 6.6 nanofibers was considerably enhanced by incorporation of 1,3,7-Trimethyl xanthine in the concentrations of 15 and 20% w/w of the polymer; which of their protection ratings were defined as "excellent protection" for both.