Determination of Ethylmalonic Acid in Urine by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

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Öztekin N., Özçelik S., Kıykım E.

First International Congress on Analytical and Bioanalytical Chemistry, Antalya, Turkey, 27 - 30 March 2019, pp.169-170

  • Publication Type: Conference Paper / Full Text
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.169-170
  • Istanbul Technical University Affiliated: Yes


Ethylmalonic acid (EMA) is a biochemical metabolite for disorders that are seen in inborn error metabolism. This metabolite accumulates in blood or urine. We developed an inexpensive and simple capillary electrophoretic (CE) with capacitively coupled contactless conductivity detection (C4D) method for the quantitative determination of EMA in urine samples. The electrophoretic conditions were optimized using 50 mM MES-Tris and 0.14 mM CTAB at pH of 6.5 as buffer solution. The separation was carried out at reversed polarity mode using a cationic surfactant as the buffer additive. Under these conditions urine samples were directly injected to the capillary without any pretreatment step. The analytical parameters of the method as linearity, precision, and detection and quantification limits were also investigated. The proposed method was applied to the determination of EMA in urine samples.