A low-cost, portable, and practical LAMP device for point-of-diagnosis in the field


Kaymaz S. V. , Ergenç A. F. , AYTEKİN A. Ö. , LUCAS S., ELİTAŞ M.

BIOTECHNOLOGY AND BIOENGINEERING, vol.119, pp.994-1003, 2022 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 119
  • Publication Date: 2022
  • Doi Number: 10.1002/bit.28025
  • Title of Journal : BIOTECHNOLOGY AND BIOENGINEERING
  • Page Numbers: pp.994-1003
  • Keywords: colony-PCR, field, high-throughput, LAMP, remote control, ISOTHERMAL AMPLIFICATION REACTION, COLORIMETRIC DETECTION, RAPID DETECTION, MICRODEVICE, ASSAY, DNA

Abstract

Transition of rapid, ready-to-use, and low-cost nucleic acid-based detection technologies from laboratories to points of sample collection has drastically accelerated. However, most of these approaches are still incapable of diagnosis starting from sampling through nucleic acid isolation and detection in the field. Here we developed a simple, portable, low-cost, colorimetric, and remotely controllable platform for reliable, high-throughput, and rapid diagnosis using loop-mediated isothermal amplification (LAMP) assays. It consists of a thermally isolated cup, low-cost electronic components, a polydimethylsiloxane sample well, and a fast prototyped case that covers electronic components. The steady-state temperature error of the system is <1%. We performed LAMP, Colony-LAMP, and Colony polymerase chain reactions (PCRs) using the yaiO(2) primer set for Escherichia coli and Pseudomonas aeruginosa samples at 65 degrees C and 30 min. We detected the end-point colorimetric readouts by the naked eye under day light. We confirmed the specificity and sensitivity of our approach using pure genomic DNA and crude bacterial colonies. We benchmarked our Colony-LAMP detection against Colony PCR. The number of samples tested can easily be modified for higher throughput in our system. We strongly believe that our platform can greatly contribute rapid and reliable diagnosis in versatile operational environments.