A methionine biomolecule-modified chromenylium-cyanine fluorescent probe for the analysis of Hg2+ in the environment and living cells

Alçay Y., Özdemir E. T., Yıldırım M. S., Ertugral U., Yavuz Ö., Arıbuğa H., ...More

Talanta, vol.259, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 259
  • Publication Date: 2023
  • Doi Number: 10.1016/j.talanta.2023.124471
  • Journal Name: Talanta
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, L'Année philologique, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Chromenylium cyanine, Density functional theory, Hazardous, Mercury, Near-IR probe, Ratiometric sensor
  • Istanbul Technical University Affiliated: Yes

Abstract

The objective of the study is, for the first time, to construct a new near infrared (NIR) fluorophore, spectrophotometric, colorimetric, ratiometric, and turn-on probe (CSME) based on chromenylium cyanine platform decorated with methionine biomolecule to provide an efficient solution for critical shortcoming to be encountered for analysis of hazardous Hg2+ in environment and living cell. The CSME structure and its interaction with Hg2+ ion were evaluated by NMR, FTIR, MS, UV–Vis and fluorescence methods as well as Density Functional Theory (DFT) calculations. The none fluorescence CSME having spirolactam ring only interacted with Hg2+ in aqueous solution including competing ions. This interaction caused the fluorescence CSME with opened spirolactam form which exhibited spectral and colorimetric changes in the NIR region. The probe based on UV–Vis and fluorescence techniques respond in 90 s, has wide linear ranges (for UV–Vis: 6.29 × 10−8 – 1.86 × 10−4 M; for fluorescence: 9.49 × 10−9 – 1.13 × 10−5 M), and has a lower Limit of Detection (LOD) value (for fluorescence: 4.93 × 10−9 M, 0.99 ng/mL) than the value predicted by the US Environmental Protection Agency (EPA) organization. Hg2+ analysis was performed in drinking and tap water with low Relative Standard Deviation (RSD) values and high recovery. Smartphone and living cell applications were successfully performed for colorimetric sensing Hg2+ in real samples and 3T3 cells, respectively.