In this study, the moisture, thermal, and tactile comfort properties of flax and hemp blended denim fabrics treated with selected common industrial washing treatments were analyzed. Being used recently in denim fabric production, four different types of flax and hemp blended fabrics in different compositions and a reference cotton fabric were produced in similar construction and treated with rinse, stone, and bleach washes. The impact of fiber composition and the washing treatments on comfort properties was analyzed statistically. The findings revealed that the blended fabrics had better moisture comfort properties and thermal resistance than the cotton fabrics. Moreover, their tactile comfort properties were within acceptable limits. The washing treatments improved the comfort properties of all fabrics in a similar way.