Modelling temperature and pH dependence of lipase and peroxidase activity in Turkish hazelnuts

Seyhan F., Tijskens L., Evranuz O.

JOURNAL OF FOOD ENGINEERING, cilt.52, sa.4, ss.387-395, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 4
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0260-8774(01)00130-3
  • Dergi Adı: JOURNAL OF FOOD ENGINEERING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.387-395
  • İstanbul Teknik Üniversitesi Adresli: Hayır

Özet

The activity of lipase and peroxidase in Turkish hazelnuts was measured experimentally as a function of temperature at a constant pH of 4.5, and as a function of pH at a constant temperature of 25 degreesC. Lipase revealed an iso-enzyme system with two clearly distinguishable peaks in activity with pH, whereas peroxidase only showed one large peak of activity. The activity data obtained were analysed by non-linear regression using an existing model for enzyme activity as a function of pH and temperature simultaneously. The existing model was adapted to account for the iso-enzyme system found to be present in hazelnut lipase activity. The percentage variances accounted for (R-adj(2)) obtained for peroxidase were 85.6% and 96.2% for temperature and pH dependence, respectively. For lipase the percentages Were found to be 72.1% and 89.6%, respectively. These results corroborate significantly the previously developed fundamental model. (C) 2002 Elsevier Science Ltd. All rights reserved.