This study intended to evaluate the effect of gender, exerting type, and temperature on the Muscle fatigue (the MVC difference between pre-task and post-task, denoted by Delta MVC), the muscle fatigue rate (%Delta MVC), the difference between pre-task and post-task time to reach MVC (Delta T-MVC), and the Cumulative 30 sec exertion output (CEO) of grip and pinch. There were totally 12 right-handed volunteers (6 males, and 6 females), and a nested-factorial design with Subject nested within gender was employed. The ANOVA result showed that the effects of gender, temperature, and exerting type affected the fatigue significantly. Males had a better muscular performance due to the greater MVC, CEO, and the same decrease in % Delta MVC and Delta T-MVC. On average, male Delta MVC was 6.15kg, with a decrement rate of 23% (6.15/26.84); Female Delta MVC was 3.68kg, with a decrement rate of 19% (3.68/18.98). In addition, the grip CEO much greater than pinch CEO, and pinch CEO was averagely about 28% (184.3 kgw*s /651.1 kgw*s) of grip CEO. For the temperature effect, the average 34 degrees C Delta MVC was 5.82kg, with a decrement rate of 25% (5.82/22.58); 12 degrees C Delta MVC was 4.00kg, with a decrement rate of 18% (4.00/18.98). In other word, after warm immersion more muscle fibers could be recruited at once during the cumulative 30 see exertion period and than lead to a greater muscle fatigue at least.