Baker's yeast industry has significant effects on environment due to significant water consumption and high strength wastewater production. Effluents from baker's yeast industry are characterized by high concentrations of biological oxygen demand (BOD(5)), chemical oxygen demand (COD), total Kjeldahl nitrogen (TKN), total dissolved solids (TDS), sulfate, and dark brown color. The aim of this study is to provide a detailed quantitative and qualitative identification and characterization of the wastewater sources from different processes of a baker's yeast industry. It is identified that the most polluted wastewater components of the facility are coming from rotary drum filter and evaporation process. However, the highest pollution loads is identified as tank and equipment cleaning effluent due to very high flow rate from this source. The efforts should be on the way to minimize the water usage for the tank and equipment cleaning process, and to recycle less polluted waste streams back to the process.