Structural analysis and properties of dextran produced by Weissella confusa and the effect of different cereals on its rheological characteristics

Heperkan Z. D. , Bolluk M. , Bulbul S.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.143, pp.305-313, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 143
  • Publication Date: 2020
  • Doi Number: 10.1016/j.ijbiomac.2019.12.036
  • Page Numbers: pp.305-313


Weissella confuse is a commonly found species in boza, a highly viscous beverage obtained from fermented cereals. Exopolysaccharide (EPS) produced by Weissella confusa C19 was characterized and the role of different cereals on its rheological characteristics were studied. Thus, the effect of the type of cereals on textural characteristics of boza for standard boza production and the fate of W. confusa during fermentation were assessed. W. confuse C19 EPS consisted of glucose, sucrose and 1.7 mg/kg protein. Structural characterization of water-soluble dextran was determined by H-1 NMR, functional groups were identified by Fourier transform infrared spectroscopy (FT-IR) and the crystal structure was determined by X-ray diffraction (XRD). After EPS characterization, different cereal-based media including maize, oat, rice and wheat were used for the growth and EPS production of the bacterium. The rheologial properties of EPS obtained from different cereal-based media showed that the steady shear behavior of EPS was pseudoplastic. W. confuse C19 is a unique strain that can adapt to the environment containing high sugar to produce high amounts of polysaccharides. Besides new information on EPS from W. confuse origin, this study showed that the amount of dextran increased using solid media fortified with different cereals. (C) 2019 Elsevier B.V. All rights reserved.